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Clinical trials to determine the impacts of using anabolic steroids in human beings began approximately three years later after the first steroid was synthesizedin the late 1940s. However, before this time, there was extensive work done on muscle breakdown as well as a number of studies on anabolic steroid administration. The study of steroid use and human performance has been characterized by two types of experimental design. In the first type of study , the researchers utilized the use of laboratory animals to study the effect of long-term steroid exposure in humans. The purpose of these studies was to determine whether long-term administration of anabolic steroids would lead to impairments in human performance. In the second type of experimental design , researchers used human subjects who had undergone drug-related brain surgery for brain cancer to determine whether anabolic steroids caused a reduction in brain function. These two type of experimental design were designed to test the effect of long-term steroid exposure on brain function and muscle functioning in human subjects. Since the advent of steroid research from the 1940s, long-term and drug-related steroid exposure has been investigated in human subjects to obtain evidence of the effects of long-term steroid use on brain function and muscle function. The use of experimental designs has been utilized to test anabolic steroid-induced decrements in brain function and muscle function in laboratory animals [16–19]. Several studies involving human subjects have found that drug-related brain injuries, including those involving the brainstem, have not only impaired human performance but also led to decrements in mood, behavior and brain function. Moreover, this research indicates that drug-induced alterations in brain function and muscle function in human subjects may result from long-term steroid exposure and may not be reversible. These findings, which have led to a decrease in the level of a hormone that affects human performance and mood, would suggest that long-term steroid exposure may negatively influence human performance and mood. Thus, the effects of anabolic steroid use on brain function and muscle function may be reversible and have no effects on mood and behavior.The effects of long-term steroid exposure on body composition and strength have also been investigated . Long-term steroid use has been linked to weight gain and fat deposition [21–23]. Therefore, these two types of laboratory animals research have provided evidence that steroid use induces changes in body composition and strength in laboratory animals . These types of researchers have also demonstrated the effects of long-term steroid exposure on body composition and strength in human subjects. Therefore, the effects of use on testosterone and sex hormone levels in human subjects are a possible source of a decrease in strength  and body mass [Related Article: